Course Content
Zambian Paediatric & Obstetrics-Gynecology (OB/GYN) Clinical Mastery

Rhabdomyosarcoma (RMS)

 Description

Rhabdomyosarcoma (RMS) is an embryonal malignancy derived from primitive mesenchymal cells, the same embryologic origin as striated muscle. It belongs to the small round blue cell tumour family based on light microscopy.

  • Represents ~50% of all pediatric soft tissue sarcomas

  • Two-thirds occur in children <6 years

  • Slightly more common in males

WHO 2013 Histological Classification

  • Embryonal RMS (ERMS) – fusion-negative

  • Alveolar RMS (ARMS) – associated with PAX-FOXO1 fusions

  • Pleomorphic RMS (PRMS)

  • Spindle cell/Sclerosing RMS (SRMS)

Genetic Notes

  • 60% of ARMS: PAX3-FOXO1, t(2;13)

  • 20% of ARMS: PAX7-FOXO1, t(1;13)

  • Remaining ARMS: rare or fusion-negative

  • ERMS: fusion-negative

  • Extremity tumours have the worst prognosis

 Anatomical Distribution and Patterns of Spread

Primary Sites, Frequencies, and Metastatic Patterns

Primary Site Frequency Metastatic Pattern
Head and neck 34%
Orbit 8%
Non-parameningeal 8%
Parameningeal 18% CNS
Genitourinary 25%
Paratesticular/Vagina/Uterus 12% Lymphatic
Bladder/Prostate 11%
Extremity 15% Lymphatic + Haematogenous
Other sites 25%

Abbreviations: CNS = Central nervous system; GU = Genitourinary; NPM = Non-parameningeal; PM = Parameningeal

 Investigations

A. Imaging

  • MRI of primary site + lymph nodes

  • CT of primary site + lymph nodes

  • CT chest

  • PET scan or bone scan or skeletal survey for staging

B. Biopsy & Laboratory Tests

  • Biopsy of primary tumour

    • Immunohistochemistry: MyoD1, Desmin, Myogenin, MSA

  • Bilateral bone marrow aspirate/biopsy

  • Lumbar puncture for CSF cytology (PM/paraspinal tumours)

  • RT-PCR & FISH using FOXO1 probe for PAX-FOXO1 fusions

  • FBC, LFTs, Urea, Creatinine

 Treatment

Risk-adapted therapy

  • Chemotherapy with VAC regimen

  • Alternating VDC with IE

  • Total duration: 39 weeks (13 VDC/IE cycles)

VAC Regimen

Drug Dose Route Schedule
Vincristine 1.5 mg/m² IV push Day 1
Actinomycin D 0.05 mg/kg IV push Day 1
Cyclophosphamide 1200 mg/m² IV infusion (≥30 mins) Day 1 + MESNA 20% of dose IV ×3 doses q4h

VDC Regimen 

Drug Dose Route Frequency Schedule
Vincristine 1.5 mg/m² IV OD Day 1, 8, 15
Doxorubicin 30 mg/m² IV OD Day 1, 2
Cyclophosphamide 600 mg/m² IV OD Day 1, 2

IE Regimen 

Drug Dose Route Frequency Schedule
Ifosfamide 1800 mg/m² IV OD Day 1–5
MESNA 20% of total Ifosfamide dose IV Every 4 hours ×3 Day 1–5
Etoposide 100 mg/m² IV OD Day 1–5
Filgrastim 5 mcg/kg/day SC OD Day 7–14

Palliative Oral Metronomic Therapy (12 cycles)

Drug Dose Schedule
Oral Etoposide 50 mg/m² daily 3 weeks on → 7–10 days off
Oral Cyclophosphamide 2.5 mg/kg daily 3 weeks on → 7–10 days off
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